Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PDK4 Rabbit pAb (A13337)

Publications (8) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PDK4 Rabbit pAb (A13337)

Western blot analysis of various lysates, using PDK4 antibody (A13337) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded rat heart using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded human placenta using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded mouse heart using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PDK4 Rabbit pAb (A13337)

Immunofluorescence analysis of U2OS cells using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name PDK4 Rabbit pAb
Catalog No. A13337
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 102-411 of human PDK4 (NP_002603.1).
Sequence FHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM
Gene ID 5166
Swiss prot Q16654
Synonyms PDK4
Calculated MW 46kDa
Observed MW 46kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse skeletal muscle, Rat liver, Rat skeletal muscle
Cellular location Mitochondrion matrix
Customer validation

WB (Mus musculus, Homo sapiens)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PDK4 Rabbit pAb images

ABclonal:Western blot - PDK4 Rabbit pAb (A13337)}

Western blot - PDK4 Rabbit pAb (A13337)

Western blot analysis of various lysates, using PDK4 antibody (A13337) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)}

Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded rat heart using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)}

Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded human placenta using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PDK4 Rabbit pAb (A13337)}

Immunohistochemistry - PDK4 Rabbit pAb (A13337)

Immunohistochemistry analysis of paraffin-embedded mouse heart using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PDK4 Rabbit pAb (A13337)}

Immunofluorescence - PDK4 Rabbit pAb (A13337)

Immunofluorescence analysis of U2OS cells using PDK4 Rabbit pAb (A13337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PDK4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PDK4. (Distance between topics and target gene indicate popularity.) PDK4

* Data provided by citexs.com, for reference only.

Publishing research using A13337? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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