Products > Catalog Antibodies > Polyclonal Antibodies

PCNA Polyclonal Antibody (A0264)

Publications (3) Datasheet

ABclonal:Western blot - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Overview

Product namePCNA Polyclonal Antibody
Catalog No.A0264
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1).
SequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
Gene ID5111
Swiss protP12004
SynonymsATLD2
Calculated MW28kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA549, MCF7, Jurkat, HeLa, HepG2, Mouse brain, Rat spinal cord
Cellular locationNucleus
Customer validation

IHC(Human)

WB(Human, Mouse)

ABclonal:Western blot - PCNA Polyclonal Antibody (A0264) }

Western blot - PCNA Polyclonal Antibody (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded human colon using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded human gastric using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse lung using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Polyclonal Antibody (A0264) }

Immunohistochemistry - PCNA Polyclonal Antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse ovary using PCNA antibody (A0264) at dilution of 1:100 (40x lens).

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