Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PARP1 Rabbit pAb (A0010)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded rat testis using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded human tonsil using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse kidney using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of THP-1 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of L929 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of C6 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PARP1 Rabbit pAb
Catalog No. A0010
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 662-1014 of PARP1 (NP_001609.2).
Sequence KSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW
Gene ID 142
Swiss prot P09874
Synonyms PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; PARP1
Calculated MW 113kDa
Observed MW 116kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293F, Mouse placenta
Cellular location Nucleus, nucleolus
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

PARP1 Rabbit pAb images

ABclonal:Western blot - PARP1 Rabbit pAb (A0010)}

Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded rat testis using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded human tonsil using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse kidney using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of THP-1 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of L929 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of C6 cells using PARP1 Rabbit pAb (A0010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PARP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PARP1. (Distance between topics and target gene indicate popularity.) PARP1

* Data provided by citexs.com, for reference only.

Publishing research using A0010? Please let us know so that we can cite the reference in this datasheet.

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