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PAK4 Polyclonal Antibody (A2782)

Publication (1) Datasheet

ABclonal:Western blot - PAK4 Polyclonal Antibody (A2782)

Overview

Product namePAK4 Polyclonal Antibody
Catalog No.A2782
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenRecombinant protein of human PAK4
SequenceEmail for sequence
Gene ID10298
Swiss protO96013
SynonymsPAK4; p21 (RAC1) activated kinase 4
Calculated MW47kDa/48kDa/54kDa/64kDa
Observed MW64kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW480, BT-474, HT-29, HeLa, 293T
Cellular locationCytoplasm
Customer validation

WB(Human)

ABclonal:Western blot - PAK4 Polyclonal Antibody (A2782) }

Western blot - PAK4 Polyclonal Antibody (A2782)

Western blot analysis of extracts of various cell lines, using PAK4 antibody (A2782) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 50s.

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