Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

P2RY2 Rabbit pAb (A13923)

Publications (2) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - P2RY2 Rabbit pAb (A13923)

Western blot analysis of extracts of various cell lines, using P2RY2 antibody (A13923) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name P2RY2 Rabbit pAb
Catalog No. A13923
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 278-377 of human P2RY2 (NP_788086.1).
Sequence CHTLNAINMAYKVTRPLASANSCLDPVLYFLAGQRLVRFARDAKPPTGPSPATPARRRLGLRRSDRTDMQRIEDVLGSSEDSRRTESTPAGSENTKDIRL
Gene ID 5029
Swiss prot P41231
Synonyms P2U; HP2U; P2U1; P2UR; P2Y2; P2RU1; P2Y2R; P2RY2
Calculated MW 42kDa
Observed MW 42-52kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MCF7, HL-60, THP-1, SKOV3, BT-474, Mouse liver, Mouse heart, Mouse skeletal muscle
Cellular location Cell membrane, Multi-pass membrane protein
Customer validation

IHC (Mus musculus)

WB (Homo sapiens)

Research Area

P2RY2 Rabbit pAb images

ABclonal:Western blot - P2RY2 Rabbit pAb (A13923)}

Western blot - P2RY2 Rabbit pAb (A13923)

Western blot analysis of extracts of various cell lines, using P2RY2 antibody (A13923) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on P2RY2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to P2RY2. (Distance between topics and target gene indicate popularity.) P2RY2

* Data provided by citexs.com, for reference only.

Publishing research using A13923? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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