Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

P2RX2 Rabbit pAb (A7078)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - P2RX2 Rabbit pAb (A7078)

Western blot analysis of extracts of various cell lines, using P2RX2 antibody (A7078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name P2RX2 Rabbit pAb
Catalog No. A7078
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human P2RX2 (NP_733782.1).
Sequence VFIVQKSYQESETGPESSIITKVKGITTSEHKVWDVEEYVKPPEGGSVFSIITRVEATHSQTQGTCPESIRVHNATCLSDADCVAGELDMLGNGLRTGRCVPYYQGPSKTCEVFGWCPVEDGASVSQFLGTMAPNFTILIKNSIHYPKFHFSKGNIADRTDGYLKRCTFHEASDLYCPIFKLGFIVEKAGESFTELAHKGGVIGVIINWDCDLDLPASECNPKYSFRRLDPKHVPASSGYNFRFAKYYKINGTTTRTLIKA
Gene ID 22953
Swiss prot Q9UBL9
Synonyms P2X2; DFNA41; P2RX2
Calculated MW 52kDa
Observed MW 50-52kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples MCF7, BT-474, HepG2, HL-60, Mouse liver, Mouse testis, Mouse heart, Rat liver, Rat heart
Cellular location Cell membrane, Multi-pass membrane protein
Customer validation

WB (Rattus norvegicus)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

P2RX2 Rabbit pAb images

ABclonal:Western blot - P2RX2 Rabbit pAb (A7078)}

Western blot - P2RX2 Rabbit pAb (A7078)

Western blot analysis of extracts of various cell lines, using P2RX2 antibody (A7078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on P2RX2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to P2RX2. (Distance between topics and target gene indicate popularity.) P2RX2

* Data provided by citexs.com, for reference only.

Publishing research using A7078? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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