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Occludin Polyclonal Antibody (A2601)

Review (2)Publication (3) Datasheet

ABclonal:Western blot - Occludin Polyclonal Antibody (A2601)
ABclonal:Immunohistochemistry - Occludin Polyclonal Antibody (A2601)
ABclonal:Immunohistochemistry - Occludin Polyclonal Antibody (A2601)
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601)
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601)
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601)
Review
ABclonal: review for Occludin Polyclonal Antibody(A2601)
Review
ABclonal: review for Occludin Polyclonal Antibody(A2601)

Overview

Product nameOccludin Polyclonal Antibody
Catalog No.A2601
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
SequenceFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
Gene ID100506658
Swiss protQ16625
SynonymsOCLN; BLCPMG; PPP1R115; PTORCH1; occludin; Occludin
Calculated MW8kDa/23kDa/31kDa/52kDa/54kDa/59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse brain, Mouse kidney, Mouse liver, Rat liver
Cellular locationCell junction, Membrane, Multi-pass membrane protein, tight junction
Customer validation

(, )

WB(Mouse, Rat)

ABclonal:Western blot - Occludin Polyclonal Antibody (A2601) }

Western blot - Occludin Polyclonal Antibody (A2601)

Western blot analysis of extracts of various cell lines, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Occludin Polyclonal Antibody (A2601) }

Immunohistochemistry - Occludin Polyclonal Antibody (A2601)

Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (A2601) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Occludin Polyclonal Antibody (A2601) }

Immunohistochemistry - Occludin Polyclonal Antibody (A2601)

Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (A2601) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601) }

Immunofluorescence - Occludin Polyclonal Antibody (A2601)

Immunofluorescence analysis of HeLa cells using Occludin antibody (A2601) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601) }

Immunofluorescence - Occludin Polyclonal Antibody (A2601)

Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (A2601) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Occludin Polyclonal Antibody (A2601) }

Immunofluorescence - Occludin Polyclonal Antibody (A2601)

Immunofluorescence analysis of PC12 cells using Occludin antibody (A2601) at dilution of 1:100. Blue: DAPI for nuclear staining.

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