Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ODF2 Rabbit pAb (A15630)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ODF2 Rabbit pAb (A15630)

Western blot analysis of various lysates using ODF2 Rabbit pAb (A15630) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - ODF2 Rabbit pAb (A15630)

Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (A15630) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ODF2 Rabbit pAb (A15630)

Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (A15630) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name ODF2 Rabbit pAb
Catalog No. A15630
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 630-829 of human ODF2 (NP_702911.1).
Sequence IISDLRSRIEHQGDKLEMAREKHQASQKENKQLSLKVDELERKLEATSAQNIEFLQVIAKREEAIHQSQLRLEEKTRECGTLARQLESAIEDARRQVEQTKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA
Gene ID 4957
Swiss prot Q5BJF6
Synonyms CT134; ODF84; ODF2/1; ODF2/2; ODF2
Calculated MW 95kDa
Observed MW 80kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HepG2, Jurkat, 293T, Mouse brain, Mouse lung, Mouse testis, Rat testis
Cellular location centriolar subdistal appendage, centriole, centrosome, ciliary basal body, ciliary transition fiber, cilium, cytosol, nucleus, sperm flagellum, spindle pole
Customer validation

WB (Mus musculus, Homo sapiens)

IF (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ODF2 Rabbit pAb images

ABclonal:Western blot - ODF2 Rabbit pAb (A15630)}

Western blot - ODF2 Rabbit pAb (A15630)

Western blot analysis of various lysates using ODF2 Rabbit pAb (A15630) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - ODF2 Rabbit pAb (A15630)}

Immunofluorescence - ODF2 Rabbit pAb (A15630)

Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (A15630) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ODF2 Rabbit pAb (A15630)}

Immunofluorescence - ODF2 Rabbit pAb (A15630)

Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb (A15630) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ODF2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ODF2. (Distance between topics and target gene indicate popularity.) ODF2

* Data provided by citexs.com, for reference only.

Publishing research using A15630? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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