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NRF1 Rabbit pAb (A14190)

Datasheet

ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)
ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)
ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190)
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190)
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190)

Overview

Product nameNRF1 Rabbit pAb
Catalog No.A14190
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2)-like 1' which has an official symbol of NFE2L1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NRF1 (NP_001035199.1).
SequenceMEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHL
Gene ID4899
Swiss protQ16656
SynonymsNRF1; ALPHA-PAL
Calculated MW36kDa/46kDa/53kDa/55kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus
ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190) }

Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)

Immunohistochemistry of paraffin-embedded human liver damage using NRF1 Antibody (A14190) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190) }

Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)

Immunohistochemistry of paraffin-embedded mouse brain using NRF1 Antibody (A14190) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NRF1 Polyclonal Antibody (A14190) }

Immunohistochemistry - NRF1 Polyclonal Antibody (A14190)

Immunohistochemistry of paraffin-embedded mouse kidney using NRF1 Antibody (A14190) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190) }

Immunofluorescence - NRF1 Polyclonal Antibody (A14190)

Immunofluorescence analysis of NIH/3T3 cells using NRF1 antibody (A14190) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190) }

Immunofluorescence - NRF1 Polyclonal Antibody (A14190)

Immunofluorescence analysis of PC12 cells using NRF1 antibody (A14190) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF1 Polyclonal Antibody (A14190) }

Immunofluorescence - NRF1 Polyclonal Antibody (A14190)

Immunofluorescence analysis of HeLa cells using NRF1 antibody (A14190) at dilution of 1:100. Blue: DAPI for nuclear staining.

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