Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NAT10 Rabbit pAb (A7292)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - NAT10 Rabbit pAb (A7292)

Western blot analysis of extracts of various cell lines, using NAT10 antibody (A7292) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ABclonal:Immunofluorescence - NAT10 Rabbit pAb (A7292)

Immunofluorescence analysis of MCF-7 cells using NAT10 antibody (A7292). Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name NAT10 Rabbit pAb
Catalog No. A7292
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NAT10 (NP_078938.2).
Sequence MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGV
Gene ID 55226
Swiss prot Q9H0A0
Synonyms ALP; Kre33; NET43; NAT10
Calculated MW 116kDa
Observed MW 116kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HepG2, HeLa
Cellular location Nucleus, nucleolus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NAT10 Rabbit pAb images

ABclonal:Western blot - NAT10 Rabbit pAb (A7292)}

Western blot - NAT10 Rabbit pAb (A7292)

Western blot analysis of extracts of various cell lines, using NAT10 antibody (A7292) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
ABclonal:Immunofluorescence - NAT10 Rabbit pAb (A7292)}

Immunofluorescence - NAT10 Rabbit pAb (A7292)

Immunofluorescence analysis of MCF-7 cells using NAT10 antibody (A7292). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A7292 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on NAT10. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NAT10. (Distance between topics and target gene indicate popularity.) NAT10

* Data provided by citexs.com, for reference only.

Publishing research using A7292? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order