Products > Catalog Antibodies > Tag and Loading Control Antibodies

Plant actin Monoclonal Antibody (AC009)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009)
ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009)

Overview

Product namePlant actin Monoclonal Antibody
Catalog No.AC009
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant Protein of plants actin
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW45kDa
Observed MW45kDa
ReactivityVarious Plants
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesArabidopsis thaliana tissue, Arabidopsis thaliana, Oryza sativa, Cassava, Zea mays
Cellular location
Customer validation

WB(Zea mays,Arabidopsis thaliana,Tomato, Poplar, unknown, Brassica napus)

ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009) }

Western blot - Plant actin Monoclonal Antibody (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using plants actin antibody (AC009).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - Plant actin Monoclonal Antibody (AC009) }

Western blot - Plant actin Monoclonal Antibody (AC009)

Western blot analysis of extracts of various cell lines, using Plants actin antibody (AC009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Submit an review for AC009

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about AC009 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AC009? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order