Product Type > Antibodies > Tag and Loading Control Antibodies

Mouse anti Trx-Tag mAb (AE023)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - Mouse anti Trx-Tag mAb (AE023)

Western blot analysis of over-expressed Trx fusion protein using Trx-tag antibody (AE023) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Mouse anti Trx-Tag mAb
Catalog No. AE023
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0511

Background

Thioredoxin is a class of small redox proteins known to be present in all organisms. It plays a role in many important biological processes, including redox signaling. In humans, thioredoxins are encoded by TXN and TXN2 genes. Loss-of-function mutation of either of the two human thioredoxin genes is lethal at the four-cell stage of the developing embryo. Although not entirely understood, thioredoxin plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). In plants, thioredoxins regulate a spectrum of critical functions, ranging from photosynthesis to growth, flowering and the development and germination of seeds. It has also recently been found to play a role in cell-to-cell communication.

Immunogen information

Immunogen Recombinant protein of Trx tag
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms TRX; TRX tag; TRX-tag
Calculated MW
Observed MW Refer to Figures

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location
Customer validation

WB (Shrimp)

Mouse anti Trx-Tag mAb images

ABclonal:Western blot - Mouse anti Trx-Tag mAb (AE023)}

Western blot - Mouse anti Trx-Tag mAb (AE023)

Western blot analysis of over-expressed Trx fusion protein using Trx-tag antibody (AE023) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on TRX. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TRX. (Distance between topics and target gene indicate popularity.) TRX

* Data provided by citexs.com, for reference only.

Publishing research using AE023? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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