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Mouse anti Myc-Tag mAb (AE010)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - Myc-Tag antibody (AE010)
ABclonal:Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)
ABclonal:Immunoprecipitation - Myc-tag antibody (AE010)

Overview

Product nameMouse anti Myc-Tag mAb
Catalog No.AE010
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide of Myc-Tag
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:2000 - 1:5000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

IF(HepG2)

WB(Mouse, Human)

ABclonal:Western blot - Myc-Tag antibody (AE010)}

Western blot - Myc-Tag antibody (AE010)

Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-Tag antibody (AE010).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)}

Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)

Immunofluorescence analysis of 293T cells using Mouse anti Myc-Tag mAb (AE010). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Myc-tag antibody (AE010)}

Immunoprecipitation - Myc-tag antibody (AE010)

Immunoprecipitation of over-expressed Myc-tagged protein in 293T cells incubated using Myc-tag antibody (AE010). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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