Products > Catalog Antibodies > Tag and Loading Control Antibodies

Mouse anti HA-Tag mAb (AE008)

Review (1)Publications (12) Datasheet

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008)
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)
ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)

Overview

Product nameMouse anti HA-Tag mAb
Catalog No.AE008
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide of YPYDVPDYA (HA-tag)-KLH
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:2000 - 1:5000
IF 1:50 - 1:500
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(unknow, Human, Mouse, Pig)

IHC(Mouse)

CoIP(Oryza Sativa)

IP(Human)

ABclonal:Western blot - Mouse anti HA-Tag mAb (AE008) }

Western blot - Mouse anti HA-Tag mAb (AE008)

Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008) }

Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb (AE008). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Mouse anti HA-Tag mAb (AE008) }

Immunoprecipitation - Mouse anti HA-Tag mAb (AE008)

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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