Products > Catalog Antibodies > Tag and Loading Control Antibodies

Mouse anti DDDDK-Tag mAb (AE005)

Publications (10) Datasheet

ABclonal:Western blot - DDDDK-tag antibody (AE005)
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)
ABclonal:Immunoprecipitation - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)

Overview

Product nameMouse anti DDDDK-Tag mAb
Catalog No.AE005
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic Peptide of human DDDDK-Tag
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:2000 - 1:5000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(Human, Pig, Mouse, Plant, Arabidopsis thaliana)

IF(Human)

CoIP(Oryza Sativa)

ChIP(Mouse)

ABclonal:Western blot - DDDDK-tag antibody (AE005)}

Western blot - DDDDK-tag antibody (AE005)

Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK-tag antibody (AE005) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)}

Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (AE005). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)}

Immunoprecipitation - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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