Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MYH9 Rabbit pAb (A0173)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - MYH9 Rabbit pAb (A0173)

Western blot analysis of extracts of various cell lines, using MYH9 antibody (A0173) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunohistochemistry - MYH9 Rabbit pAb (A0173)

Immunohistochemistry analysis of paraffin-embedded human esophagus using MYH9 antibody (A0173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MYH9 Rabbit pAb (A0173)

Immunohistochemistry analysis of paraffin-embedded human stomach using MYH9 antibody (A0173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name MYH9 Rabbit pAb
Catalog No. A0173
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9 (NP_002464.1).
Sequence ANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE
Gene ID 4627
Swiss prot P35579
Synonyms MHA; FTNS; EPSTS; BDPLT6; DFNA17; MATINS; NMMHCA; NMHC-II-A; NMMHC-IIA; MYH9
Calculated MW 227kDa
Observed MW 250kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, Jurkat, LO2, SW480, Mouse liver, Mouse lung
Cellular location Cytoplasm, cell cortex, cytoskeleton
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

MYH9 Rabbit pAb images

ABclonal:Western blot - MYH9 Rabbit pAb (A0173)}

Western blot - MYH9 Rabbit pAb (A0173)

Western blot analysis of extracts of various cell lines, using MYH9 antibody (A0173) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - MYH9 Rabbit pAb (A0173)}

Immunohistochemistry - MYH9 Rabbit pAb (A0173)

Immunohistochemistry analysis of paraffin-embedded human esophagus using MYH9 antibody (A0173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MYH9 Rabbit pAb (A0173)}

Immunohistochemistry - MYH9 Rabbit pAb (A0173)

Immunohistochemistry analysis of paraffin-embedded human stomach using MYH9 antibody (A0173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on MYH9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYH9. (Distance between topics and target gene indicate popularity.) MYH9

* Data provided by citexs.com, for reference only.

Publishing research using A0173? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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