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MYH11 Rabbit pAb (A10827)


ABclonal:Western blot - MYH11 Polyclonal Antibody (A10827)
ABclonal:Immunofluorescence - MYH11 Polyclonal Antibody (A10827)


Product nameMYH11 Rabbit pAb
Catalog No.A10827
Host speciesRabbit
Purification methodAffinity purification
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1680-1979 of human MYH11 (NP_001035203.1).
Gene ID4629
Swiss protP35749
SynonymsMYH11; AAT4; FAA4; SMHC; SMMHC; myosin-11
Calculated MW223kDa/224kDa/227kDa/228kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesLO2, A375, Mouse liver, Mouse brain, Rat liver
Cellular locationMelanosome
ABclonal:Western blot - MYH11 Polyclonal Antibody (A10827) }

Western blot - MYH11 Polyclonal Antibody (A10827)

Western blot analysis of extracts of various cell lines, using MYH11 antibody (A10827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - MYH11 Polyclonal Antibody (A10827) }

Immunofluorescence - MYH11 Polyclonal Antibody (A10827)

Immunofluorescence analysis of human placenta cells using MYH11 antibody (A10827) at dilution of 1:100. Blue: DAPI for nuclear staining.

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