Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MTTP Rabbit pAb (A1746)

Reviews (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - MTTP Rabbit pAb (A1746)

Western blot analysis of extracts of various cell lines, using MTTP antibody (A1746) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - MTTP Rabbit pAb (A1746)

Immunofluorescence analysis of NIH/3T3 cells using MTTP Rabbit pAb (A1746) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for MTTP Rabbit pAb(A1746)
Review
ABclonal: review for MTTP Rabbit pAb(A1746)

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Overview

Product name MTTP Rabbit pAb
Catalog No. A1746
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MTTP (NP_000244.2).
Sequence SKIVRRVLKEMVAHNYDRF
Gene ID 4547
Swiss prot P55157
Synonyms ABL; MTP; MTTP
Calculated MW 99kDa
Observed MW 99kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse liver, Rat small intestine
Cellular location Endoplasmic reticulum
Customer validation

WB (rat liver, Mouse liver)

Documents

Certificate of Compliance

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Lot number

Research Area

MTTP Rabbit pAb images

ABclonal:Western blot - MTTP Rabbit pAb (A1746)}

Western blot - MTTP Rabbit pAb (A1746)

Western blot analysis of extracts of various cell lines, using MTTP antibody (A1746) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MTTP Rabbit pAb (A1746)}

Immunofluorescence - MTTP Rabbit pAb (A1746)

Immunofluorescence analysis of NIH/3T3 cells using MTTP Rabbit pAb (A1746) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MTTP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MTTP. (Distance between topics and target gene indicate popularity.) MTTP

* Data provided by citexs.com, for reference only.

Publishing research using A1746? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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