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MTOR Polyclonal Antibody (A2445)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - MTOR antibody (A2445)
ABclonal:Immunofluorescence - MTOR antibody (A2445)
ABclonal:Immunofluorescence - MTOR antibody (A2445)
ABclonal:Immunofluorescence - MTOR antibody (A2445)
ABclonal:Immunoprecipitation - 3ug MTOR antibody (A2445)

Overview

Product nameMTOR Polyclonal Antibody
Catalog No.A2445
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MTOR (NP_004949.1).
SequenceMLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
Gene ID2475
Swiss protP42345
SynonymsFRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS
Calculated MW288kDa
Observed MW260kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, HepG2, K-562, Mouse kidney, Mouse brain, Rat brain
Cellular locationCytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

WB(unknow, Pig)

ABclonal:Western blot - MTOR antibody (A2445)}

Western blot - MTOR antibody (A2445)

Western blot analysis of extracts of various cell lines, using MTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MTOR antibody (A2445)}

Immunofluorescence - MTOR antibody (A2445)

Immunofluorescence analysis of HeLa cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MTOR antibody (A2445)}

Immunofluorescence - MTOR antibody (A2445)

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MTOR antibody (A2445)}

Immunofluorescence - MTOR antibody (A2445)

Immunofluorescence analysis of PC12 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - 3ug MTOR antibody (A2445)}

Immunoprecipitation - 3ug MTOR antibody (A2445)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug MTOR antibody (A2445). Western blot was performed from the immunoprecipitate using MTOR antibody (A2445) at a dilition of 1:1000.

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