Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MRE11 Rabbit pAb (A2559)

Publications (7) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - MRE11 Rabbit pAb (A2559)

Western blot analysis of extracts of K-562 cells, using MRE11 antibody (A2559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - MRE11 Rabbit pAb (A2559)

Western blot analysis of extracts of various cell lines, using MRE11 antibody (A2559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MRE11 Rabbit pAb (A2559)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MRE11 Rabbit pAb (A2559) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MRE11 Rabbit pAb (A2559)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using MRE11 Rabbit pAb (A2559) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name MRE11 Rabbit pAb
Catalog No. A2559
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human MRE11 (NP_005582.1).
Sequence MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLH
Gene ID 4361
Swiss prot P49959
Synonyms ATLD; HNGS1; MRE11A; MRE11B; MRE11
Calculated MW 81kDa
Observed MW 81kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples K-562, Neuro-2a, Mouse liver, Mouse brain
Cellular location Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

MRE11 Rabbit pAb images

ABclonal:Western blot - MRE11 Rabbit pAb (A2559)}

Western blot - MRE11 Rabbit pAb (A2559)

Western blot analysis of extracts of K-562 cells, using MRE11 antibody (A2559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - MRE11 Rabbit pAb (A2559)}

Western blot - MRE11 Rabbit pAb (A2559)

Western blot analysis of extracts of various cell lines, using MRE11 antibody (A2559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MRE11 Rabbit pAb (A2559)}

Immunohistochemistry - MRE11 Rabbit pAb (A2559)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MRE11 Rabbit pAb (A2559) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MRE11 Rabbit pAb (A2559)}

Immunohistochemistry - MRE11 Rabbit pAb (A2559)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using MRE11 Rabbit pAb (A2559) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on MRE11. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MRE11. (Distance between topics and target gene indicate popularity.) MRE11

* Data provided by citexs.com, for reference only.

Publishing research using A2559? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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