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MMP1 Polyclonal Antibody (A1191)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - MMP1 Polyclonal Antibody (A1191)
ABclonal:Immunohistochemistry - MMP1 Polyclonal Antibody (A1191)
ABclonal:Immunohistochemistry - MMP1 Polyclonal Antibody (A1191)
Review
ABclonal: review for MMP1 Polyclonal Antibody(A1191)

Overview

Product nameMMP1 Polyclonal Antibody
Catalog No.A1191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human MMP1 (NP_002412.1).
SequenceFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDS
Gene ID4312
Swiss protP03956
SynonymsMMP1; CLG; CLGN; interstitial collagenase
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSKOV3, HT-29
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Mouse)

ABclonal:Western blot - MMP1 Polyclonal Antibody (A1191) }

Western blot - MMP1 Polyclonal Antibody (A1191)

Western blot analysis of extracts of various cell lines, using MMP1 antibody (A1191) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MMP1 Polyclonal Antibody (A1191) }

Immunohistochemistry - MMP1 Polyclonal Antibody (A1191)

Immunohistochemistry of paraffin-embedded human cervical carcinoma using MMP1 antibody (A1191) at dilution of 1:300 (40x lens).
ABclonal:Immunohistochemistry - MMP1 Polyclonal Antibody (A1191) }

Immunohistochemistry - MMP1 Polyclonal Antibody (A1191)

Immunohistochemistry of paraffin-embedded mouse kidney using MMP1 antibody (A1191) at dilution of 1:200 (40x lens).

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