Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MMP11 Rabbit pAb (A3034)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MMP11 Rabbit pAb (A3034)

Western blot analysis of extracts of various cell lines, using MMP11 antibody (A3034) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

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Overview

Product name MMP11 Rabbit pAb
Catalog No. A3034
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 239-488 of human MMP11 (NP_005931.2).
Sequence FRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPDAPPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDAQGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADGYAYFLRGRLYWKFDPVKVKALEGFPRLVGPDFFGCAEPANTFL
Gene ID 4320
Swiss prot P24347
Synonyms ST3; SL-3; STMY3; MMP11
Calculated MW 55kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Raji, 293T, Mouse lung, Mouse kidney, Mouse skeletal muscle, Mouse ovary, Mouse heart
Cellular location Secreted, extracellular matrix, extracellular space
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

MMP11 Rabbit pAb images

ABclonal:Western blot - MMP11 Rabbit pAb (A3034)}

Western blot - MMP11 Rabbit pAb (A3034)

Western blot analysis of extracts of various cell lines, using MMP11 antibody (A3034) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

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Related Targets

Based on existing publications, the following genes are closely related to research on MMP11. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MMP11. (Distance between topics and target gene indicate popularity.) MMP11

* Data provided by citexs.com, for reference only.

Publishing research using A3034? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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