Catalog No:A2094
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - MKI67 antibody (A2094)
ABclonal:Immunofluorescence - MKI67 antibody (A2094)

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This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1200-1300 of human MKI67 (NP_001139438.1).
Gene ID4288
Swiss ProtP46013
SynonymsKIA; MIB-; MIB-1; PPP1R105
Calculated MW319kDa/358kDa
Observed MW360kDa


Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular LocationChromosome, Nucleus, nucleolus
Customer Validation

IHC (Mouse, Human)

IF (Mouse)

MKI67 Polyclonal Antibody images

ABclonal:Western blot - MKI67 antibody (A2094)

Western blot - MKI67 antibody (A2094)

Western blot analysis of extracts of HeLa cells, using MKI67 antibody (A2094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MKI67 antibody (A2094)

Immunofluorescence - MKI67 antibody (A2094)

Immunofluorescence analysis of HeLa cells using MKI67 antibody (A2094). Blue: DAPI for nuclear staining.

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