Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

MDM2 Mouse mAb (A0346)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - MDM2 Mouse mAb (A0346)

Western blot analysis of extracts of MCF-7 cells, using MDM2 antibody (A0346).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name MDM2 Mouse mAb
Catalog No. A0346
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Immunogen information

Immunogen Recombinant protein of human MDM2
Sequence Email for sequence
Gene ID 4193
Swiss prot Q00987
Synonyms HDMX; LSKB; hdm2; ACTFS; MDM2
Calculated MW 55kDa
Observed MW 58kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples MCF7
Cellular location Cytoplasm, Nucleus, nucleolus, nucleoplasm

Research Area

MDM2 Mouse mAb images

ABclonal:Western blot - MDM2 Mouse mAb (A0346)}

Western blot - MDM2 Mouse mAb (A0346)

Western blot analysis of extracts of MCF-7 cells, using MDM2 antibody (A0346).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on MDM2. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to MDM2. (Distance between topics and target gene indicate popularity.) MDM2

* Data provided by citexs.com, for reference only.

Publishing research using A0346? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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