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MAP2K6 Rabbit pAb (A2575)

Publications (2) Datasheet

ABclonal:Western blot - MAP2K6 Polyclonal Antibody (A2575)
ABclonal:Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575)


Product nameMAP2K6 Rabbit pAb
Catalog No.A2575
Host speciesRabbit
Purification methodAffinity purification
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MAP2K6 (NP_002749.2).
Gene ID5608
Swiss protP52564
Calculated MW31kDa/37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus, cytoskeleton
Customer validation

WB(Oryza sativa, Sus scrofa)

ABclonal:Western blot - MAP2K6 Polyclonal Antibody (A2575) }

Western blot - MAP2K6 Polyclonal Antibody (A2575)

Western blot analysis of extracts of HeLa cells, using MAP2K6 antibody (A2575) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575) }

Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575)

Immunofluorescence analysis of U2OS cells using MAP2K6 antibody (A2575). Blue: DAPI for nuclear staining.

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