Products > Catalog Antibodies > Polyclonal Antibodies

MAP2K6 Rabbit pAb (A2575)

Publications (2) Datasheet

ABclonal:Western blot - MAP2K6 Polyclonal Antibody (A2575)
ABclonal:Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575)

Overview

Product nameMAP2K6 Rabbit pAb
Catalog No.A2575
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human MAP2K6 (NP_002749.2).
SequenceMSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD
Gene ID5608
Swiss protP52564
SynonymsMAP2K6; MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3
Calculated MW31kDa/37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus, cytoskeleton
Customer validation

WB(Oryza sativa, Sus scrofa)

ABclonal:Western blot - MAP2K6 Polyclonal Antibody (A2575) }

Western blot - MAP2K6 Polyclonal Antibody (A2575)

Western blot analysis of extracts of HeLa cells, using MAP2K6 antibody (A2575) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575) }

Immunofluorescence - MAP2K6 Polyclonal Antibody (A2575)

Immunofluorescence analysis of U2OS cells using MAP2K6 antibody (A2575). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A2575 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A2575? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Contact us to order