Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MAGEA6 Rabbit pAb (A8130)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - MAGEA6 Rabbit pAb (A8130)

Western blot analysis of extracts of various cell lines, using MAGEA6 antibody (A8130) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - MAGEA6 Rabbit pAb (A8130)

Immunofluorescence analysis of U2OS cells using MAGEA6 antibody (A8130) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name MAGEA6 Rabbit pAb
Catalog No. A8130
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 75-314 of human MAGEA6 (NP_005354.1).
Sequence MNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAKLVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASDSLQLVFGIELMEVDPIGHVYIFATCLGLSYDGLLGDNQIMPKTGFLIIILAIIAKEGDCAPEEKIWEELSVLEVFEGREDSIFGDPKKLLTQYFVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHMVKISGGPRISYPLLHEWALREGEE
Gene ID 4105
Swiss prot P43360
Synonyms CT1.6; MAGE6; MAGE3B; MAGE-3b; MAGEA6
Calculated MW 35kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 22Rv1, Mouse heart
Cellular location nucleus

Documents

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Research Area

MAGEA6 Rabbit pAb images

ABclonal:Western blot - MAGEA6 Rabbit pAb (A8130)}

Western blot - MAGEA6 Rabbit pAb (A8130)

Western blot analysis of extracts of various cell lines, using MAGEA6 antibody (A8130) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MAGEA6 Rabbit pAb (A8130)}

Immunofluorescence - MAGEA6 Rabbit pAb (A8130)

Immunofluorescence analysis of U2OS cells using MAGEA6 antibody (A8130) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAGEA6. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAGEA6. (Distance between topics and target gene indicate popularity.) MAGEA6

* Data provided by citexs.com, for reference only.

Publishing research using A8130? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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