Products > Catalog Antibodies > Polyclonal Antibodies

Lamin A/C Polyclonal Antibody (A0249)

Reviews (4)Publications (11) Datasheet

ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Overview

Product nameLamin A/C Polyclonal Antibody
Catalog No.A0249
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
SequenceSSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHVSGSRR
Gene ID4000
Swiss protP02545
SynonymsCDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; PRO1
Calculated MW62-74kDa
Observed MW74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, THP-1
Cellular locationNucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation

WB(Primary mouse liver, U251, Human cells, Mouse splenocytes)

ELISA(Mouse)

ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249) }

Western blot - Lamin A/C Polyclonal Antibody (A0249)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A0249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded human kidney cancer using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human colon using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human esophageal using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human gastric using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human kidney using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human kidney cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human liver injury using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded human liver cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Rat heart using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A0249). Blue: DAPI for nuclear staining.

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