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LRRC59 Polyclonal Antibody (A10022)

Datasheet

ABclonal:Western blot - LRRC59 Polyclonal Antibody (A10022)

Overview

Product nameLRRC59 Polyclonal Antibody
Catalog No.A10022
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human LRRC59 (NP_060979.2).
SequenceMTKAGSKGGNLRDKLDGNELDLSLSDLNEVPVKELAALPKATILDLSCNKLTTLPSDFCGLTHLVKLDLSKNKLQQLPADFGRLVNLQHLDLLNNKLVTLPVSFAQLKNLKWLDLKDNPLDPVLAKVAGDCLDEKQCKQCANKVLQHMKAVQADQERERQRRLEVEREAEKKREAKQRAKEAQERELRKREKAEEKERRRKEYDALKAAKREQEKKPKKEANQAPKSKSGSRPRKPPPRKHTRS
Gene ID55379
Swiss protQ96AG4
SynonymsLRRC59; PRO1855; p34
Calculated MW34kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:4000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, SH-SY5Y, BT-474, HepG2, NIH/3T3, Mouse liver, Rat liver
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Nucleus envelope, Single-pass type II membrane protein
ABclonal:Western blot - LRRC59 Polyclonal Antibody (A10022) }

Western blot - LRRC59 Polyclonal Antibody (A10022)

Western blot analysis of extracts of various cell lines, using LRRC59 antibody (A10022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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