Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

LNPEP Rabbit pAb (A11959)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - LNPEP Rabbit pAb (A11959)

Western blot analysis of lysates from Mouse brain, using LNPEP Rabbit pAb (A11959) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - LNPEP Rabbit pAb (A11959)

Western blot analysis of lysates from Rat brain, using LNPEP Rabbit pAb (A11959) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded rat liver using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded rat brain using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded human placenta using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name LNPEP Rabbit pAb
Catalog No. A11959
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human LNPEP (NP_005566.2).
Sequence MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSART
Gene ID 4012
Swiss prot Q9UIQ6
Synonyms CAP; IRAP; PLAP; P-LAP; LNPEP
Calculated MW 117kDa
Observed MW 165kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse brain,Rat brain
Cellular location Cell membrane, Secreted, Single-pass type II membrane protein
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

LNPEP Rabbit pAb images

ABclonal:Western blot - LNPEP Rabbit pAb (A11959)}

Western blot - LNPEP Rabbit pAb (A11959)

Western blot analysis of lysates from Mouse brain, using LNPEP Rabbit pAb (A11959) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - LNPEP Rabbit pAb (A11959)}

Western blot - LNPEP Rabbit pAb (A11959)

Western blot analysis of lysates from Rat brain, using LNPEP Rabbit pAb (A11959) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)}

Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded rat liver using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)}

Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded rat brain using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LNPEP Rabbit pAb (A11959)}

Immunohistochemistry - LNPEP Rabbit pAb (A11959)

Immunohistochemistry analysis of LNPEP in paraffin-embedded human placenta using LNPEP Rabbit pAb (A11959) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on LNPEP. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to LNPEP. (Distance between topics and target gene indicate popularity.) LNPEP

* Data provided by citexs.com, for reference only.

Publishing research using A11959? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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