Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

LC3B Rabbit pAb (A11923)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - LC3B Rabbit pAb (A11923)

Western blot analysis of lysates from 293 cells, using LC3B Rabbit pAb (A11923).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - LC3B Rabbit pAb (A11923)

Western blot analysis of various lysates using LC3B Rabbit pAb (A11923) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded rat brain using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded human colon using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded mouse brain using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name LC3B Rabbit pAb
Catalog No. A11923
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
Sequence MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYE
Gene ID 81631
Swiss prot Q9GZQ8
Synonyms LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Calculated MW 15kDa
Observed MW 20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293
Cellular location Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

IHC (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

LC3B Rabbit pAb images

ABclonal:Western blot - LC3B Rabbit pAb (A11923)}

Western blot - LC3B Rabbit pAb (A11923)

Western blot analysis of lysates from 293 cells, using LC3B Rabbit pAb (A11923).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - LC3B Rabbit pAb (A11923)}

Western blot - LC3B Rabbit pAb (A11923)

Western blot analysis of various lysates using LC3B Rabbit pAb (A11923) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)}

Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded rat brain using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)}

Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded human colon using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LC3B Rabbit pAb (A11923)}

Immunohistochemistry - LC3B Rabbit pAb (A11923)

Immunohistochemistry analysis of LC3B in paraffin-embedded mouse brain using LC3B Rabbit pAb (A11923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAP1LC3B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAP1LC3B. (Distance between topics and target gene indicate popularity.) MAP1LC3B

* Data provided by citexs.com, for reference only.

Publishing research using A11923? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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