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L3MBTL3 Polyclonal Antibody (A7289)

Review (1) Datasheet

ABclonal:Western blot - L3MBTL3 Polyclonal Antibody (A7289)
ABclonal:Immunofluorescence - L3MBTL3 Polyclonal Antibody (A7289)
ABclonal:Immunoprecipitation - L3MBTL3 Polyclonal Antibody (A7289)
Review
ABclonal: review for L3MBTL3 Polyclonal Antibody(A7289)

Overview

Product nameL3MBTL3 Polyclonal Antibody
Catalog No.A7289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
SequenceMTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDENEMENVKKATATTTWMVPTAQEVFSEKTGMPFRLKDPVKVEGLQFCENCCQYGNVDECLSGGNYCSQNCARHIKDKDQKEERDVEEDNEEEDPKCSRKKKPKLSLKADTKEDGEERDDEMENKQDVRILRGSQRARRKRRGDSAVLKQGLPPKGKKAWCWASYLEEEKAVAVPAKLFKEHQ
Gene ID84456
Swiss protQ96JM7
SynonymsL3MBTL3; MBT-1; MBT1; l(3)mbt-like 3 (Drosophila)
Calculated MW85kDa/88kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, U-251MG, Mouse spleen
Cellular locationNucleus
Customer validation

ABclonal:Western blot - L3MBTL3 Polyclonal Antibody (A7289) }

Western blot - L3MBTL3 Polyclonal Antibody (A7289)

Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody (A7289) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
ABclonal:Immunofluorescence - L3MBTL3 Polyclonal Antibody (A7289) }

Immunofluorescence - L3MBTL3 Polyclonal Antibody (A7289)

Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (A7289). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - L3MBTL3 Polyclonal Antibody (A7289) }

Immunoprecipitation - L3MBTL3 Polyclonal Antibody (A7289)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (A7289). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (A7289) at a dilition of 1:1000.

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