Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

KRT36 Rabbit pAb (A13759)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - KRT36 Rabbit pAb (A13759)

Western blot analysis of various lysates using (A13759) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - KRT36 Rabbit pAb (A13759)

Immunohistochemistry analysis of KRT36 in paraffin-embedded human lung cancer using KRT36 Rabbit pAb (A13759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name KRT36 Rabbit pAb
Catalog No. A13759
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KRT36 (NP_003762.1).
Sequence LNDRLANYLEKVRQLERENAELESRIQEWYEFQIPYICPDYQSYFKTIEDFQQKILLTKSENARLVLQIDNAKLAADDFRTKYETELSLRQLVEADINGLR
Gene ID 8689
Swiss prot O76013
Synonyms HA6; hHa6; KRTHA6; KRT36
Calculated MW 52kDa
Observed MW 52kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Raji, Rat testis
Cellular location cytoskeleton, cytosol, extracellular exosome, intermediate filament cytoskeleton

Documents

Certificate of Compliance

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Research Area

KRT36 Rabbit pAb images

ABclonal:Western blot - KRT36 Rabbit pAb (A13759)}

Western blot - KRT36 Rabbit pAb (A13759)

Western blot analysis of various lysates using (A13759) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - KRT36 Rabbit pAb (A13759)}

Immunohistochemistry - KRT36 Rabbit pAb (A13759)

Immunohistochemistry analysis of KRT36 in paraffin-embedded human lung cancer using KRT36 Rabbit pAb (A13759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on KRT36. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KRT36. (Distance between topics and target gene indicate popularity.) KRT36

* Data provided by citexs.com, for reference only.

Publishing research using A13759? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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