Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Western blot analysis of extracts of various cell lines, using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Immunofluorescence analysis of A-549 cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at dilution of 1:200. Blue: DAPI for nuclear staining.

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Overview

Product name Cytokeratin 17 (KRT17) Rabbit pAb
Catalog No. A2175
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 133-432 of human Cytokeratin 17 (Cytokeratin 17 (KRT17)) (NP_000413.1).
Sequence QYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
Gene ID 3872
Swiss prot Q04695
Synonyms PC; K17; PC2; 39.1; CK-17; PCHC1; Cytokeratin 17 (KRT17)
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, HepG2, OVCAR3
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Cytokeratin 17 (KRT17) Rabbit pAb images

ABclonal:Western blot - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)}

Western blot - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Western blot analysis of extracts of various cell lines, using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)}

Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Immunofluorescence analysis of A-549 cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)}

Immunofluorescence - Cytokeratin 17 (KRT17) Rabbit pAb (A2175)

Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (Cytokeratin 17 (KRT17)) antibody (A2175) at dilution of 1:200. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on KRT17. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KRT17. (Distance between topics and target gene indicate popularity.) KRT17

* Data provided by citexs.com, for reference only.

Publishing research using A2175? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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