Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

[KD Validated] TRAF2 Rabbit mAb (A19129)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of lysates from wild type(WT) and TRAF2 knockdown (KD) 293T cells, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - [KD Validated] TRAF2 Rabbit mAb (A19129)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TRAF2 antibody (A19129). Western blot was performed from the immunoprecipitate using TRAF2 antibody (A19129) at a dilution of 1:50000.

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Overview

Product name [KD Validated] TRAF2 Rabbit mAb
Catalog No. A19129
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC51292

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human TRAF2 (NP_066961.2).
Sequence DGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY
Gene ID 7186
Swiss prot Q12933
Synonyms TRAP; TRAP3; RNF117; MGC:45012; [KD Validated] TRAF2
Calculated MW 53kDa/54kDa/55kDa/61kDa
Observed MW 53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P Human
WB HumanMouseRat
Recommended dilution
  • WB 1:10000 - 1:60000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples 293T, HeLa, C2C12, Mouse thymus, Rat thymus
Cellular location Cytoplasm
Customer validation

WB (Homo sapiens, Sus scrofa, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] TRAF2 Rabbit mAb images

ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)}

Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)}

Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)}

Western blot - [KD Validated] TRAF2 Rabbit mAb (A19129)

Western blot analysis of lysates from wild type(WT) and TRAF2 knockdown (KD) 293T cells, using [KD Validated] TRAF2 Rabbit mAb (A19129) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - [KD Validated] TRAF2 Rabbit mAb (A19129)}

Immunoprecipitation - [KD Validated] TRAF2 Rabbit mAb (A19129)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TRAF2 antibody (A19129). Western blot was performed from the immunoprecipitate using TRAF2 antibody (A19129) at a dilution of 1:50000.

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Related Targets

Based on existing publications, the following genes are closely related to research on TRAF2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TRAF2. (Distance between topics and target gene indicate popularity.) TRAF2

* Data provided by citexs.com, for reference only.

Publishing research using A19129? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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