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[KO Validated] SIRT1 Rabbit pAb (A11267)

Publications (10) Datasheet

ABclonal:Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267)

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (A11267) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267)

Western blot analysis of extracts of Mouse liver, using SIRT1 antibody (A11267) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunohistochemistry of paraffin-embedded human colon using [KO Validated] SIRT1 Rabbit pAb (A11267) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunofluorescence analysis of HeLa cells using SIRT1 antibody (A11267) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (A11267). Western blot was performed from the immunoprecipitate using SIRT1 antibody (A11267) at a dilition of 1:1000.

Overview

Product name[KO Validated] SIRT1 Rabbit pAb
Catalog No.A11267
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
SequenceVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS
Gene ID23411
Swiss protQ96EB6
SynonymsSIR2; SIR2L1; SIR2alpha; SIRT1; sirtuin 1
Calculated MW61kDa/81kDa
Observed MW120KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Mouse liver
Cellular locationCytoplasm, Mitochondrion, Nucleus, Nucleus, PML body
Customer validation

WB(Mus musculus, Rattus norvegicus, Danio rerio, Homo sapiens, Sus scrofa)

ChIP(Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267) }

Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267)

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (A11267) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267) }

Western blot - [KO Validated] SIRT1 Rabbit pAb (A11267)

Western blot analysis of extracts of Mouse liver, using SIRT1 antibody (A11267) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] SIRT1 Rabbit pAb (A11267) }

Immunohistochemistry - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunohistochemistry of paraffin-embedded human colon using [KO Validated] SIRT1 Rabbit pAb (A11267) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SIRT1 Rabbit pAb (A11267) }

Immunofluorescence - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunofluorescence analysis of HeLa cells using SIRT1 antibody (A11267) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SIRT1 Rabbit pAb (A11267) }

Immunoprecipitation - [KO Validated] SIRT1 Rabbit pAb (A11267)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (A11267). Western blot was performed from the immunoprecipitate using SIRT1 antibody (A11267) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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