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[KO Validated] RELA Polyclonal Antibody (A11201)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] RELA Polyclonal Antibody (A11201)
ABclonal:Western blot - [KO Validated] RELA Polyclonal Antibody (A11201)
ABclonal:Immunohistochemistry - [KO Validated] RELA Polyclonal Antibody (A11201)
ABclonal:Immunofluorescence - [KO Validated] RELA Polyclonal Antibody (A11201)

Overview

Product name[KO Validated] RELA Polyclonal Antibody
Catalog No.A11201
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-550 of human RELA (NP_068810.3).
SequenceLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQIS
Gene ID5970
Swiss protQ04206
SynonymsRELA; NFKB3; p65; transcription factor p65
Calculated MW58kDa/59kDa/60kDa
Observed MW75kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mouse)

ABclonal:Western blot - [KO Validated] RELA Polyclonal Antibody (A11201) }

Western blot - [KO Validated] RELA Polyclonal Antibody (A11201)

Western blot analysis of extracts of HeLa cells, using RELA antibody (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] RELA Polyclonal Antibody (A11201) }

Western blot - [KO Validated] RELA Polyclonal Antibody (A11201)

Western blot analysis of extracts from normal (control) and RELA knockout (KO) HeLa cells, using RELA antibody (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] RELA Polyclonal Antibody (A11201) }

Immunohistochemistry - [KO Validated] RELA Polyclonal Antibody (A11201)

Immunohistochemistry of paraffin-embedded human breast cancer using RELA Antibody (A11201) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] RELA Polyclonal Antibody (A11201) }

Immunofluorescence - [KO Validated] RELA Polyclonal Antibody (A11201)

Immunofluorescence analysis of A549 cells using RELA antibody (A11201) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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