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[KO Validated] RAB27A Rabbit pAb (A1934)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] RAB27A Rabbit pAb (A1934)

Western blot analysis of extracts of U-937 cells, using RAB27A antibody (A1934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

ABclonal:Western blot - [KO Validated] RAB27A Rabbit pAb (A1934)

Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (A1934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934)

Immunofluorescence analysis of L929 cells using RAB27A antibody (A1934) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934)

Immunofluorescence analysis of U-2 OS cells using RAB27A antibody (A1934) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] RAB27A Rabbit pAb
Catalog No.A1934
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).
SequenceMSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC
Gene ID5873
Swiss protP51159
SynonymsRAB27A; GS2; HsT18676; RAB27; RAM
Calculated MW24kDa
Observed MW25kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesU-937
Cellular locationLate endosome, Lipid-anchor, Lysosome, Melanosome, Membrane
Customer validation

WB(Homo sapiens,Mus musculus)

IF(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] RAB27A Rabbit pAb (A1934) }

Western blot - [KO Validated] RAB27A Rabbit pAb (A1934)

Western blot analysis of extracts of U-937 cells, using RAB27A antibody (A1934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] RAB27A Rabbit pAb (A1934) }

Western blot - [KO Validated] RAB27A Rabbit pAb (A1934)

Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (A1934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934) }

Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934)

Immunofluorescence analysis of L929 cells using RAB27A antibody (A1934) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934) }

Immunofluorescence - [KO Validated] RAB27A Rabbit pAb (A1934)

Immunofluorescence analysis of U-2 OS cells using RAB27A antibody (A1934) at dilution of 1:100. Blue: DAPI for nuclear staining.

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