Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PD-L1/CD274 Rabbit pAb (A21443)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - PD-L1/CD274 Rabbit pAb (A21443)

Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody (A21443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name PD-L1/CD274 Rabbit pAb
Catalog No. A21443
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PD-L1/CD274 (NP_054862.1).
Sequence LFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
Gene ID 29126
Swiss prot Q9NZQ7
Synonyms B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; PD-L1/CD274
Calculated MW 33kDa
Observed MW 40kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2
Cellular location Cell membrane, Endomembrane system, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Research Area

PD-L1/CD274 Rabbit pAb images

ABclonal:Western blot - PD-L1/CD274 Rabbit pAb (A21443)}

Western blot - PD-L1/CD274 Rabbit pAb (A21443)

Western blot analysis of extracts of HepG2 cells, using PD-L1/CD274 antibody (A21443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CD274. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CD274. (Distance between topics and target gene indicate popularity.) CD274

* Data provided by citexs.com, for reference only.

Publishing research using A21443? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

Proteins (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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