Western blot - PARP1 Rabbit mAb (A19596)
Western blot analysis of lysates from Jurkat cells, using PARP1 Rabbit mAb (A19596) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - PARP1 Rabbit mAb (A19596)
Western blot analysis of various lysates using PARP1 Rabbit mAb (A19596) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded human colon using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded human liver cancer using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded human placenta using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded human spleen using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded human tonsil using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse brain using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse colon using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse kidney using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded mouse testis using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded rat brain using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded rat heart using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - PARP1 Rabbit mAb (A19596)
Immunohistochemistry analysis of PARP1 in paraffin-embedded rat liver using PARP1 Rabbit mAb (A19596) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - PARP1 Rabbit mAb (A19596)
Immunofluorescence analysis of HeLa cells using PARP1 Rabbit mAb (A19596) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - PARP1 Rabbit mAb (A19596)
Immunofluorescence analysis of paraffin-embedded human lung using PARP1 Rabbit mAb (A19596) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.