Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] CD73/NT5E Rabbit pAb (A2029)

KO/KDValidated

Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of lysates from Rat eye, using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of lysates from wild type (WT) and CD73/NT5E knockout (KO) HCT116 cells, using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human liver cancer using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name [KO Validated] CD73/NT5E Rabbit pAb
Catalog No. A2029
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-377 of human CD73/NT5E (NP_002517.1).
Sequence WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTD
Gene ID 4907
Swiss prot P21589
Synonyms NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; 5E
Calculated MW 63kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HT-29, A375, Mouse kidney, Rat eye
Cellular location Cell membrane, GPI-anchor, Lipid-anchor
Customer validation

FC (Homo sapiens)

WB (Mus musculus)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] CD73/NT5E Rabbit pAb images

ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)}

Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)}

Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of lysates from Rat eye, using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)}

Western blot - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Western blot analysis of lysates from wild type (WT) and CD73/NT5E knockout (KO) HCT116 cells, using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)}

Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human liver cancer using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)}

Immunohistochemistry - [KO Validated] CD73/NT5E Rabbit pAb (A2029)

Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit pAb (A2029) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on NT5E. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NT5E. (Distance between topics and target gene indicate popularity.) NT5E

* Data provided by citexs.com, for reference only.

Publishing research using A2029? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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