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[KO Validated] NFKBIA Polyclonal Antibody (A11397)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397)
ABclonal:Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397)
ABclonal:Immunofluorescence - [KO Validated] NFKBIA Polyclonal Antibody (A11397)
ABclonal:Immunoprecipitation - [KO Validated] NFKBIA Polyclonal Antibody (A11397)

Overview

Product name[KO Validated] NFKBIA Polyclonal Antibody
Catalog No.A11397
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Gene ID4792
Swiss protP25963
SynonymsNFKBIA; IKBA; MAD-3; NFKBI; NFKB inhibitor alpha
Calculated MW35kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF-7, DU145, THP-1, A-549, Mouse spleen, Mouse thymus
Cellular locationCytoplasm, Nucleus
Customer validation

IHC(unknown)

ABclonal:Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397) }

Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397)

Western blot analysis of extracts of various cell lines, using NFKBIA antibody (A11397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397) }

Western blot - [KO Validated] NFKBIA Polyclonal Antibody (A11397)

Western blot analysis of extracts from normal (control) and NFKBIA knockout (KO) 293T cells, using NFKBIA antibody (A11397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - [KO Validated] NFKBIA Polyclonal Antibody (A11397) }

Immunofluorescence - [KO Validated] NFKBIA Polyclonal Antibody (A11397)

Immunofluorescence analysis of HeLa cells using NFKBIA antibody (A11397). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] NFKBIA Polyclonal Antibody (A11397) }

Immunoprecipitation - [KO Validated] NFKBIA Polyclonal Antibody (A11397)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug NFKBIA antibody (A11397). Western blot was performed from the immunoprecipitate using NFKBIA antibody (A11397) at a dilition of 1:500.

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