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[KO Validated] IKB alpha Polyclonal Antibody (A11397)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397)
ABclonal:Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397)
ABclonal:Immunoprecipitation - [KO Validated] IKB alpha Polyclonal Antibody (A11397)

Overview

Product name[KO Validated] IKB alpha Polyclonal Antibody
Catalog No.A11397
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IKB alpha (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Gene ID4792
Swiss protP25963
SynonymsNFKBIA; IKBA; MAD-3; NFKBI; NFKB inhibitor alpha
Calculated MW35kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesMCF-7, DU145, THP-1, A-549, Mouse spleen, Mouse thymus
Cellular locationCytoplasm, Nucleus
Customer validation

IHC(unknown)

ABclonal:Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397) }

Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397)

Western blot analysis of extracts of various cell lines, using IKB alpha antibody (A11397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397) }

Western blot - [KO Validated] IKB alpha Polyclonal Antibody (A11397)

Western blot analysis of extracts from normal (control) and IKB alpha knockout (KO) 293T cells, using IKB alpha antibody (A11397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunoprecipitation - [KO Validated] IKB alpha Polyclonal Antibody (A11397) }

Immunoprecipitation - [KO Validated] IKB alpha Polyclonal Antibody (A11397)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug IKB alpha antibody (A11397). Western blot was performed from the immunoprecipitate using IKB alpha antibody (A11397) at a dilition of 1:500.

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