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[KO Validated] MMP9 Polyclonal Antibody (A2095)

Review (1)Publications (7) Datasheet

ABclonal:Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095)
ABclonal:Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095)
ABclonal:Immunofluorescence - [KO Validated] MMP9 Polyclonal Antibody (A2095)
Review
ABclonal: review for [KO Validated] MMP9 Polyclonal Antibody(A2095)

Overview

Product name[KO Validated] MMP9 Polyclonal Antibody
Catalog No.A2095
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
SequenceKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED
Gene ID4318
Swiss protP14780
SynonymsMMP9; CLG4B; GELB; MANDP2; MMP-9; matrix metallopeptidase 9
Calculated MW78kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesB-cell, MCF7, K-562, SW480, mouse liver, mouse lung, mouse thymus, 293T
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Human, Rat, Mouse)

IHC(Human)

WB(Human)

IHC(Mouse)

ABclonal:Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095) }

Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095)

Western blot analysis of extracts of various cell lines, using MMP9 antibody (A2095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095) }

Western blot - [KO Validated] MMP9 Polyclonal Antibody (A2095)

Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody (A2095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] MMP9 Polyclonal Antibody (A2095) }

Immunofluorescence - [KO Validated] MMP9 Polyclonal Antibody (A2095)

Immunofluorescence analysis of U-2OS cells using MMP9 antibody (A2095) at dilution of 1:100. Blue: DAPI for nuclear staining.

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