Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

MC1 Receptor Rabbit mAb (A11594)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MC1 Receptor Rabbit mAb (A11594)

Western blot analysis of extracts of various cell lines, using MC1 Receptor Rabbit mAb (A11594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - MC1 Receptor Rabbit mAb (A11594)

Western blot analysis of extracts of Mouse kidney, using MC1 Receptor Rabbit mAb (A11594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

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Overview

Product name MC1 Receptor Rabbit mAb
Catalog No. A11594
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0638

Background

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MC1 Receptor (Q01726).
Sequence MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLEVSISDGLFLSLGLVSLVENALVVATIAKNRNLHSPMYCFICCLALSDLLVSGSNVLETAVILL
Gene ID 4157
Swiss prot Q01726
Synonyms CMM5; MSH-R; SHEP2; MC1 Receptor
Calculated MW 35kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A549, A375, C6, MCF7, Mouse kidney
Cellular location Cell membrane

Documents

Certificate of Compliance

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Lot number

Research Area

MC1 Receptor Rabbit mAb images

ABclonal:Western blot - MC1 Receptor Rabbit mAb (A11594)}

Western blot - MC1 Receptor Rabbit mAb (A11594)

Western blot analysis of extracts of various cell lines, using MC1 Receptor Rabbit mAb (A11594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - MC1 Receptor Rabbit mAb (A11594)}

Western blot - MC1 Receptor Rabbit mAb (A11594)

Western blot analysis of extracts of Mouse kidney, using MC1 Receptor Rabbit mAb (A11594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

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Related Targets

Based on existing publications, the following genes are closely related to research on MC1R. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MC1R. (Distance between topics and target gene indicate popularity.) MC1R

* Data provided by citexs.com, for reference only.

Publishing research using A11594? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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