Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

MAD1/MAD1L1 Rabbit mAb (A5098)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)

Western blot analysis of extracts of Mouse spleen, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)

Western blot analysis of extracts of various cell lines, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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Overview

Product name MAD1/MAD1L1 Rabbit mAb
Catalog No. A5098
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC1188

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MAD1/MAD1L1 (Q9Y6D9).
Sequence QLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKED
Gene ID 8379
Swiss prot Q9Y6D9
Synonyms MAD1; MVA7; PIG9; TP53I9; TXBP181; MAD1/MAD1L1
Calculated MW 83kDa
Observed MW 83kDa

Applications

Reactivity Human, Mouse
Tested applications Testing results
WB HumanMouse
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, 293T, Mouse spleen
Cellular location Chromosome, Cytoplasm, Nucleus, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule organizing center, Spindle

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

MAD1/MAD1L1 Rabbit mAb images

ABclonal:Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)}

Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)

Western blot analysis of extracts of Mouse spleen, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)}

Western blot - MAD1/MAD1L1 Rabbit mAb (A5098)

Western blot analysis of extracts of various cell lines, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAD1L1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAD1L1. (Distance between topics and target gene indicate popularity.) MAD1L1

* Data provided by citexs.com, for reference only.

Publishing research using A5098? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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