Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

[KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)

Western blot analysis of various lysates, using KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)

Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockout (KO) 293T(KO) cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit (RM00021).
Exposure time: 30s.

You may also interested in:

Overview

Product name [KD Validated] KMT5A/SETD8 Rabbit mAb
Catalog No. A4136
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0916

Background

The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KMT5A/SETD8 (Q9NQR1).
Sequence MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMS
Gene ID 387893
Swiss prot Q9NQR1
Synonyms SET8; SET07; SETD8; PR-Set7; PR/SET07; [KO/KD Validated] KMT5A/SETD8
Calculated MW 43kDa
Observed MW 49kDa

Applications

Reactivity Human, Mouse
Tested applications Testing results
WB HumanMouse
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, HeLa, U-87MG, Mouse brain, Mouse heart
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] KMT5A/SETD8 Rabbit mAb images

ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)}

Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)

Western blot analysis of various lysates, using KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)}

Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)

Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockout (KO) 293T(KO) cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit (RM00021).
Exposure time: 30s.

Inquire About This Product

Submit your question about A4136 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on KMT5A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KMT5A. (Distance between topics and target gene indicate popularity.) KMT5A

* Data provided by citexs.com, for reference only.

Publishing research using A4136? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order