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[KO Validated] KEAP1 Rabbit pAb (A17061)

Publications (3) Datasheet

SizePrice
20μL$128$88
50μL$198$130
100μL$328$220
200μL$498$380
ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts of Jurkat cells, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts of Rat lung, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts from normal (control) and KEAP1 knockout (KO) 293T cells, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded human colon using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded mouse kidney using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded rat ovary using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).

Overview

Product name[KO Validated] KEAP1 Rabbit pAb
Catalog No.A17061
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
ImmunogenA synthetic peptide of human KEAP1.
SequenceEmail for sequence
Gene ID9817
Swiss protQ14145
SynonymsKEAP1; INrf2; KLHL19
Calculated MW69kDa
Observed MW60-64KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, Jurkat, Rat lung
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts of Jurkat cells, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts of Rat lung, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Western blot - [KO Validated] KEAP1 Rabbit pAb (A17061)

Western blot analysis of extracts from normal (control) and KEAP1 knockout (KO) 293T cells, using KEAP1 antibody (A17061) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded human colon using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded mouse kidney using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061) }

Immunohistochemistry - [KO Validated] KEAP1 Rabbit pAb (A17061)

Immunohistochemistry of paraffin-embedded rat ovary using KEAP1 Rabbit pAb (A17061) at dilution of 1:100 (40x lens).

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