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[KO Validated] GSK3β Rabbit pAb (A6164)

Publications (8) Datasheet

ABclonal:Western blot - [KO Validated] GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts from normal (control) and GSK3β knockout (KO) HeLa cells, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KO Validated] GSK3β Rabbit pAb (A6164)

Immunofluorescence analysis of HeLa cells using GSK3β antibody (A6164) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] GSK3β Rabbit pAb (A6164)

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3β antibody (A6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (A6164) at a dilition of 1:1000.

Overview

Product name[KO Validated] GSK3β Rabbit pAb
Catalog No.A6164
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3β (NP_001139628.1).
SequencePWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
Gene ID2932
Swiss protP49841
SynonymsGSK3B; gsk-3β
Calculated MW46kDa/48kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesLO2, HeLa, 22Rv1, U-251MG
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Mus musculus)

IF(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] GSK3β Rabbit pAb (A6164) }

Western blot - [KO Validated] GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] GSK3β Rabbit pAb (A6164) }

Western blot - [KO Validated] GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts from normal (control) and GSK3β knockout (KO) HeLa cells, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] GSK3β Rabbit pAb (A6164) }

Immunofluorescence - [KO Validated] GSK3β Rabbit pAb (A6164)

Immunofluorescence analysis of HeLa cells using GSK3β antibody (A6164) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] GSK3β Rabbit pAb (A6164) }

Immunoprecipitation - [KO Validated] GSK3β Rabbit pAb (A6164)

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3β antibody (A6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (A6164) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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