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GSK3β Rabbit pAb (A6164)

Publications (12) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A6164)

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3β antibody (A6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (A6164) at a dilition of 1:1000.

Overview

Product nameGSK3β Rabbit pAb
Catalog No.A6164
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-420 of GSK3β (NP_001139628.1).
SequenceLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
Gene ID2932
Swiss protP49841
SynonymsGSK3B; gsk-3β
Calculated MW46kDa/48kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesLO2, HeLa, 22Rv1, U-251MG
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Rattus norvegicus)

IF(Mus musculus)

ABclonal:Western blot - GSK3β Rabbit pAb (A6164) }

Western blot - GSK3β Rabbit pAb (A6164)

Western blot analysis of extracts of various cell lines, using GSK3β antibody (A6164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A6164) }

Immunoprecipitation - GSK3β Rabbit pAb (A6164)

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3β antibody (A6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (A6164) at a dilition of 1:1000.

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