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[KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Western blot analysis of extracts of various cell lines, using EZH2/KMT6 antibody (A11085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Western blot analysis of extracts from normal (control) and EZH2/KMT6 knockout (KO) HeLa cells, using EZH2/KMT6 antibody (A11085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Immunofluorescence analysis of U2OS cells using EZH2/KMT6 antibody (A11085).

Overview

Product name[KO Validated] EZH2/KMT6 Rabbit pAb
Catalog No.A11085
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2/KMT6 (NP_001190176.1).
SequenceMGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQ
Gene ID2146
Swiss protQ15910
SynonymsENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2; EZH2; KMT6 / EZH2
Calculated MW79kDa/81kDa/84kDa/85kDa/86kDa
Observed MW78-110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMCF7, 293T, HepG2, HeLa, SW620, Raji, Mouse testis
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085) }

Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Western blot analysis of extracts of various cell lines, using EZH2/KMT6 antibody (A11085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085) }

Western blot - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Western blot analysis of extracts from normal (control) and EZH2/KMT6 knockout (KO) HeLa cells, using EZH2/KMT6 antibody (A11085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085) }

Immunofluorescence - [KO Validated] EZH2/KMT6 Rabbit pAb (A11085)

Immunofluorescence analysis of U2OS cells using EZH2/KMT6 antibody (A11085).

* For research use only. Not for therapeutic or diagnostic purposes.

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