Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] β-Catenin Rabbit pAb (A11512)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512)

Western blot analysis of extracts of various cell lines, using β-Catenin antibody (A11512) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512)

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A11512) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded rat pancreas using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug β-Catenin antibody (A11512). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A11512) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] β-Catenin Rabbit pAb(A11512)
Review
ABclonal: review for [KO Validated] β-Catenin Rabbit pAb(A11512)

Overview

Product name[KO Validated] β-Catenin Rabbit pAb
Catalog No.A11512
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human β-Catenin (NP_001895.1).
SequenceMATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW85kDa
Observed MW92KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, C6, Mouse brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

(Adenocarcinoma human alveolar basal epithelial cells, rat cells)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Danio rerio)

IF(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512)

Western blot analysis of extracts of various cell lines, using β-Catenin antibody (A11512) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Western blot - [KO Validated] β-Catenin Rabbit pAb (A11512)

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A11512) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded rat pancreas using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit pAb (A11512) }

Immunoprecipitation - [KO Validated] β-Catenin Rabbit pAb (A11512)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug β-Catenin antibody (A11512). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A11512) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11512

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A11512 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A11512? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order