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[KO Validated] Bax Rabbit pAb (A12009)

Reviews (6)Publications (13) Datasheet

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A12009)
ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A12009)
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)
ABclonal:Immunoprecipitation - [KO Validated] Bax Rabbit pAb (A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A12009)

Overview

Product name[KO Validated] Bax Rabbit pAb
Catalog No.A12009
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID581
Swiss protQ07812
SynonymsBCL2L4; BAX
Calculated MW4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, HeLa, Raji, RAW264.7, Mouse lung, Rat lung
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

(rat cells, HUVEC-P6, BEL-7402, Mouse embryonal fibroblast cell, Human fibrosarcoma cells, Human fibrosarcoma cell line)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus)

IHC(Mus musculus)

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A12009) }

Western blot - [KO Validated] Bax Rabbit pAb (A12009)

Western blot analysis of extracts of various cell lines, using Bax antibody (A12009) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A12009) }

Western blot - [KO Validated] Bax Rabbit pAb (A12009)

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (A12009) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009) }

Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)

Immunohistochemistry of paraffin-embedded rat ovary using Bax antibody (A12009) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009) }

Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)

Immunohistochemistry of paraffin-embedded human stomach using Bax antibody (A12009) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009) }

Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A12009)

Immunohistochemistry of paraffin-embedded mouse kidney using Bax antibody (A12009) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] Bax Rabbit pAb (A12009) }

Immunoprecipitation - [KO Validated] Bax Rabbit pAb (A12009)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Bax antibody (A12009). Western blot was performed from the immunoprecipitate using Bax antibody (A12009) at a dilition of 1:1000.

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